{"id":263298,"date":"2017-01-19T10:40:25","date_gmt":"2017-01-19T14:40:25","guid":{"rendered":"http:\/\/spaceweekly.com\/?guid=178b5d07057d161d87cc494e9681f29d"},"modified":"2017-01-19T10:40:25","modified_gmt":"2017-01-19T14:40:25","slug":"crispr-meets-single-cell-sequencing-in-new-screening-method","status":"publish","type":"post","link":"https:\/\/spaceweekly.com\/?p=263298","title":{"rendered":"CRISPR meets single-cell sequencing in new screening method"},"content":{"rendered":"<p>Genome editing using CRISPR\/Cas9 &#8220;gene scissors&#8221; is a powerful tool for biological discovery and for identifying novel drug targets. In pooled CRISPR screens, a large number of cells are edited simultaneously using CRISPR guide-RNAs against thousands of different genes. Next, some of the edited cells are experimentally selected, and their guide-RNAs are counted to determine which genes are most important for the studied biological mechanism.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Genome editing using CRISPR\/Cas9 &#8220;gene scissors&#8221; is a powerful tool for biological discovery and for identifying novel drug targets. In pooled CRISPR screens, a large number of cells are edited simultaneously using CRISPR guide-RNAs against thousands o&#8230;<\/p>\n","protected":false},"author":0,"featured_media":615444,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-263298","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/263298","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=263298"}],"version-history":[{"count":1,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/263298\/revisions"}],"predecessor-version":[{"id":263299,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/263298\/revisions\/263299"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/media\/615444"}],"wp:attachment":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=263298"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=263298"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=263298"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}