{"id":566310,"date":"2019-01-10T12:29:59","date_gmt":"2019-01-10T16:29:59","guid":{"rendered":"https:\/\/www.sciencedaily.com\/releases\/2019\/01\/190110112959.htm"},"modified":"2019-01-10T12:29:59","modified_gmt":"2019-01-10T16:29:59","slug":"giving-cas9-an-on-switch-for-better-control-of-crispr-gene-editing-2","status":"publish","type":"post","link":"https:\/\/spaceweekly.com\/?p=566310","title":{"rendered":"Giving Cas9 an &#8216;on&#8217; switch for better control of CRISPR gene editing"},"content":{"rendered":"<p>Scientists have created an &#8216;on&#8217; switch for CRISPR-Cas9 that allows it to be turned on in select cells only, specifically those that have a particular protein-cutting enzyme, or protease. Viruses produce such proteases, as do cancer cells, so the Cas9 variants &#8212; called ProCas9 &#8212; could be used as sensors for viral infections or cancer. The variants were discovered by circular permutations on wild-type Cas9 designed to produce a stripped-down Cas9 tuned to human cells. <\/p>\n","protected":false},"excerpt":{"rendered":"<p>Scientists have created an &#8216;on&#8217; switch for CRISPR-Cas9 that allows it to be turned on in select cells only, specifically those that have a particular protein-cutting enzyme, or protease. Viruses produce such proteases, as do cancer cells, so the Cas9 v&#8230;<\/p>\n","protected":false},"author":61,"featured_media":615444,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-566310","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/566310","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/users\/61"}],"replies":[{"embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=566310"}],"version-history":[{"count":1,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/566310\/revisions"}],"predecessor-version":[{"id":566311,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/566310\/revisions\/566311"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/media\/615444"}],"wp:attachment":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=566310"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=566310"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=566310"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}