The CRISPR\/Cas9 system has been causing ripples in the scientific community ever since its mechanisms were reported in 2012. Commonly referred to as a genome editing tool, many scientists have found different applications for the scissor-like properties of the Cas9-protein. Researchers from the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben) have now found a way to utilise the RNA\/protein complex in a slightly different way\u2014as a cytogenetic torch. Other than in conventional in situ hybridisation, the new RNA-guided endonuclease\u2014in situ labelling-tool (RGEN-ISL) no longer requires denaturation of the DNA. The new method therefore leaves the chromatin intact, enabling investigation of the structure of the sample. Moreover, RGEN-ISL can be combined with protein-detection methods and allows real-time visualisation of the labelling-process. Whilst initially developed for plant genomes, RGEN-ISL can be used in all organisms and shows to be a promising new tool in the field of chromosome biology.<\/p>\n","protected":false},"excerpt":{"rendered":"
The CRISPR\/Cas9 system has been causing ripples in the scientific community ever since its mechanisms were reported in 2012. Commonly referred to as a genome editing tool, many scientists have found different applications for the scissor-like properties of the Cas9-protein. Researchers from the Leibniz Institute of Plant Genetics and Crop Plant Research (IPK Gatersleben) have now found a way to utilise the RNA\/protein complex in a slightly different way—as a cytogenetic torch. Other than in conventional in situ hybridisation, the new RNA-guided endonuclease—in situ labelling-tool (RGEN-ISL) no longer requires denaturation of the DNA. The new method therefore leaves the chromatin intact, enabling investigation of the structure of the sample. Moreover, RGEN-ISL can be combined with protein-detection methods and allows real-time visualisation of the labelling-process. Whilst initially developed for plant genomes, RGEN-ISL can be used in all organisms and shows to be a promising new tool in the field of chromosome biology.<\/p>\n","protected":false},"author":0,"featured_media":615444,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[],"tags":[],"class_list":["post-589465","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry"],"_links":{"self":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/589465","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/types\/post"}],"replies":[{"embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=589465"}],"version-history":[{"count":1,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/589465\/revisions"}],"predecessor-version":[{"id":589466,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/posts\/589465\/revisions\/589466"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=\/wp\/v2\/media\/615444"}],"wp:attachment":[{"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=589465"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=589465"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/spaceweekly.com\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=589465"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}