Synthetic DNA allows scientists to expand the breadth and depth of their genomic research. In this study researchers from the U.S. Department of Energy Joint Genome Institute (DOE JGI) have developed a suite of build-optimization software tools (BOOST) to streamline the design-build transition in synthetic biology engineering workflows. BOOST can automatically detect “difficult” sequences (of nucleotides) and redesign them for DNA synthesis, addressing DNA sequences with certain problematic characteristics (e.g., extreme %GC, sequence patterns, and repeats), which decrease the success rate of DNA synthesis.