In vitro fertilized (IVF) embryo transfer has become an important innovation in the agricultural sectors, such as in cattle production. Approximately half of all bovine embryos produced worldwide were derived from IVF. However, the pregnancy success rate of IVF embryos transplanted into recipients remains low. To increase the success of pregnancy, key technological issues affecting the in vitro production of embryos and the assessment of viable embryos must be addressed. This is also true for human artificial reproductive technology (ART). Generally, assessment of bovine embryo quality is performed by morphological grading on days 7 to 8 post-insemination, as recommended by the International Embryo Technology Society (IETS), but the pregnancy success rate of embryos judged as transferable is only 30 percent to 50 percent. Therefore, novel technology for noninvasively and reliably selecting viable IVF embryos has been craving.