To design safe clinical trials, it is crucial to better understand and predict gene editing outcomes in preclinical studies. Bence György and collaborators have shown that adeno-associated viruses (AAVs) can stably integrate into CRISPR-Cas9-induced double-strand breaks, in up to almost half of the therapeutically targeted cells, in vitro and in vivo in mice. The team also showed that CRISPR did not cause an increase in genome-wide integration of AAV, but only at the CRISPR-cut site.
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Source: Phys.org