Light microscopy continues to reveal the microscopic world at an ever increasing resolution. Using a new method coined COLD, scientists at the Max Planck Institute for the Science of Light in Erlangen have now visualized protein structures with a resolution of around 5 Å. COLD achieves this unprecedented resolution by operating at a temperature of minus 270 degrees Celsius. At such low temperatures, the signals of fluorescent proteins can be localized more precisely. The method thereby opens up the possibility low temperatures, the signals of fluorescent proteins can be localized more precisely. The method thereby opens up the possibility of observing structural changes of proteins associated with specific diseases.