Fluid Shifts Dilution Measurements: The 49S subjects completed their Return minus 45 (R-45) Fluid Shifts Dilution Measurements activities today, collecting saliva, blood and urine and inserting them into Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI) prior to ingesting a Sodium Bromide (NaBr) tracer. Throughout the day, the crew will perform more collections, storing them in MELFI as well. Fluid Shifts is a joint NASA-Russian experiment divided into Dilution Measurements, Baseline Imaging, and Baseline Imaging with Chibis (Lower Body Negative Pressure). The Fluid Shifts experiment investigates the causes for severe and lasting physical changes to astronaut’s eyes. Because the headward fluid shift is a hypothesized contributor to these changes, reversing this fluid shift with a lower body negative pressure device is being evaluated as a possible intervention. Results from this study may help to develop preventative measures against lasting changes in vision and to prevention of eye damage. JAXA Protein Crystal Growth (PCG) #12 Installation and Execution: The crew installed and configured the JAXA PCG#12 canisters in JEM Ryutai rack. This experiment will run until removal for return on 49S. The two canisters contain a total of 47 protein samples that were prepared by Russian and Japanese researchers from universities, national research institutes, and the private sector. The purpose of this activity is to obtain high quality protein crystals in the microgravity environment at a constant temperature for about 6 weeks. Results may contribute to the development of drugs for multidrug-resistant bacteria, Alzheimer’s disease, muscular dystrophy and periodontitis. They will also aid in the development of a blood substitute and biosensor. Orbital 7 (OA-7) Arrival Preparations: In preparation for OA-7 capture and berthing this Saturday, the crew completed the following: Reviewed Cygnus attached phase configuration, hardware, and stowage. Relocated Portable Computer System (PCS) and cables from Japanese Experiment Module Pressurized Module (JPM) Utility Outlet Panel (UOP) to the Lab UOP. Completed a Robotics Onboard Trainer (RoBOT) session to practice Capture Point hold runs and 2 meter runs. Today’s Planned Activities All activities were completed unless otherwise noted. Preparation of Reports for Roscosmos Web Site and Social Media EKON-M. Observations and photography Fluid Shifts Saliva Urine, and Blood Baseline Collection Fluid Shifts MELFI Insertion Operations Fluid Shifts Galley Water Collection Tracer Ingestion Operations Fluid Shifts Refrigerated Centrifuge Configuration MELFI Overview OBT Repair CWC-I attached to UPA SPA. KASKAD. ТБУ-В #02 thermostat connection and activation to +26 °С. Cygnus Capture Review FAGEN. ТБУ-В №4 thermostat activation to +4 °С Soyuz 735 Transfers and IMS Ops Pressurization of Elektron-VM Liquid Unit before Activation On-board Training (OBT) Cygnus Attached Phase Operations review. COSMOCARD. Preparing for and Starting 24-hr ECG Recording Fluid Shifts Urine Collection Mid Cygnus Cargo Operations Review KRISTALLIZATOR. PCG kits transfer from Soyuz 735, Inspection, and Handover to USOS KRISTALLIZATOR. Photo of PCG Kits Handover Fluid Shifts MELFI Retrieve Insertion Operations JAXA Protein Crystal Growth (PCG) Installation CONSTANTA-2. Transfer and Setup in ТБУ-В No.04 at +4 deg C Progress 435 (DC1) Stowage Ops with IMS Support Fluid Shifts Blood 3-Hour Collection Fluid Shifts Blood Collection – Operator FAGEN. Kit Transfers from Soyuz 735 FAGEN. Setup of MSK kit No.06 in ТБУ-В No.4 thermostat at +4 deg C and MSK kit No.07 kit on SM panel 328, photography Fluid Shifts Refrigerated Centrifuge Configuration Fluid Shifts Saliva 3-Hour Collection Fluid Shifts MELFI Retrieve Insertion Operations Cygnus Capture Review CASKAD. Transfer of Anabioz P/L from Soyuz 735. Bioreactor Setup in Thermostat at +26 deg C. Photography STRUKTURA. Transfer of Hardware from Soyuz 735 Fluid Shifts Refrigerated Centrifuge Spin Conclude STRUKTURA. Process Activation and Placement STRUKTURA. Photo during the Experiment and at Exposure Location Fluid Shifts MELFI Retrieve Insertion Operations CEVIS Crew Handover CALCIUM. Transfer of Calcium- Э Kits from Soyuz 735 and Setup in MRM1 CALCIUM. Experiment Session 1 T2 Crew Handover Fluid Shifts Saliva 5-Hour Collection – Subject Photo of a scuffmark left by the Active Docking Assembly Probe on the MRM2 Active Docking Assembly (АСП) Receiving Cone Fluid Shifts MELFI Retrieve Insertion Operations Fluid Shifts Blood Collection Conclude Stow Download docking cone internal surface photos to OCA Fluid Shifts Urine Collection End – Subject Fluid Shifts MELFI Insertion Operations Crew time for ISS adaptation and orientation NanoRacks Microscope-3 Procedure Review On-board Training (OBT) Cygnus Robotics Onboard Trainer (ROBoT) Session 2 Installation and Testing Voltage Converter ПН28-120 in RS Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill PCS Laptop Relocate Soyuz 735 Transfers and IMS Ops Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill ISS Crew Orientation Ghost camera setup in Cupola Cygnus Cargo Operations Conference Fluid Shifts Urine Collection Stow Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill Progress 435 (DC1) Stowage and IMS Ops Health Maintenance System (HMS) – ESA Nutritional Assessment Regenerative Environmental Control and Life Support System (ECLSS) Recycle Tank Fill Part 3 IMS Update Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill Completed Task List Items Veggie 03 Pillow Watering and Photo Lab Aft Hatch Stowage Latch Troubleshooting Ground Activities All activities were completed unless otherwise noted. Cygnus OBT support Fluid Shifts support Three-Day Look Ahead: Saturday, 04/22: Cygnus capture/berth/ingress Sunday, 04/23: Crew off duty Monday, 04/24: Polar (4) transfers, Emergency Roles/Responsibilities Review, OBT Cygnus emergency review, Cygnus cargo transfer ops QUICK ISS Status – Environmental Control Group: Component Status Elektron Off Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) Off Carbon Dioxide Removal Assembly (CDRA) Lab Operate Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Operate Major Constituent Analyzer (MCA) Node 3 Standby Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Standby Trace Contaminant Control System (TCCS) Lab Full Up Trace Contaminant Control System (TCCS) Node 3 Off