OsteoOmics: The crew thawed BioCells and injected them into growth media inside the Microgravity Science Glovebox (MSG). After all six BioCells are processed were placed into BioCell Habitat 2 which is then placed in the Space Automated Bioproduct Lab (SABL). Later in the day the crew removed a media kit from a Microgravity Experiment Research Locker / INcubator (MERLIN) and inserted it into an ambient Cargo Transfer Bag for the next day’s operations. This is the first of four weeks of OsteoOmics operations. Crewmembers experience bone loss in orbit, stemming from the lack of gravity acting on their bones. OsteoOmics investigates the molecular mechanisms that dictate this bone loss by examining osteoblasts, which form bone, and osteoclasts, which dissolve bone. Improved understanding of these mechanisms could lead to more effective countermeasures to prevent bone loss during space missions and in a wide range of disorders on Earth. This may lead to better preventative care or therapeutic treatments for people suffering bone loss as a result of bone diseases like osteopenia and osteoporosis, or for patients on prolonged bed rest. Fluidics: The crew replaced fluid tanks and performed the third and final Fluidics run today. Following completion of the run the crew deinstalled the Fluidics hardware from the seat-tracks in the Columbus module. Fluidics is a fluid mechanics experiment with two main objectives: a Slosh Study to investigate fluid behavior under microgravity during satellite maneuvers, and a Wave Turbulence Study to investigate the impact of capillary effect on wave turbulence without being masked by the effect of gravity. Two tanks with different filling rations (50% and 75%) for Slosh and one tank with water for Wave Turbulence are used. Sprint Ultrasound 2 Operations: The crew set up a camera and video, configured Ultrasound 2, placed reference marks on the calf and thigh of right leg, donned Sprint (Integrated Resistance and Aerobic Training Study) thigh and calf guides, and performed thigh and calf scans with guidance from the Sprint ground team. Ultrasound scans are used to evaluate spaceflight-induced changes in the muscle volume. The Sprint investigation evaluates the use of high intensity, low volume exercise training to minimize loss of muscle, bone, and cardiovascular function in ISS crewmembers during long-duration missions. Meteor Hard Drive Swap: The crew changed out the Meteor hard drive located in the Window Observational Research Facility (WORF). The Meteor payload is a visible spectroscopy instrument with the primary purpose of observing meteors in Earth orbit. Meteor uses image analysis to provide information on the physical and chemical properties of the meteoroid dust, such as size, density, and chemical composition. Since the parent comets or asteroids for most of the meteor showers are identified, the study of the meteoroid dust on orbit provides information about the parent comets and asteroids. On-Board Training (OBT) ISS Emergency Hardware: The 49S crew completed this regularly scheduled training to re-familiarize themselves with locations of equipment and positions of valves used in emergencies. During training, the crew will consult with specialists at Mission Control Center (MCC)-Houston, MCC-Moscow, Columbus Control Center and Space Station Integrated Promotion Center (SSIPC). External Thermal Control System (ETCS) Non-propulsive Vent: Ground controllers performed a planned ETCS Loop B Radiator Beam Valve Module (RBVM) P1-3-2 vent. The ammonia vent was performed in an effort to isolate a slow leak suspected on ETCS Loop B. Today’s Planned Activities All activities were completed unless otherwise noted. On MCC GO Regeneration of Micropurification Unit (БМП) Ф2 Cartridge (start) DAN. Experiment Ops. Fluidics Run 3 execution OsteoOmics MSG Operations ALGOMETRIYA. Measurement Prep and Ops in Strain- and Thermo-Algometry Modes ISS Crew Orientation Crew time for ISS adaptation and orientation Electrical Power System Contingency Jumper Installation Big Picture Words Review Recharging Soyuz 733 Samsung PC Battery (if charge level is below 80%) Integrated Emergency Procedure Review Strata Payload Deactivation Fluidics Closeout Activities Electrical Power System Contingency Jumper Installation Big Picture Words Review Ultrasound 2 HRF Rack 1 Power On Ultrasound 2 Configuration System Settings Backup [Deferred] CASKAD. Manual Mixing in Bioreactor ALGOMETRIYA. Strain Measurements Mode Data Transmission Radio Link (RSPI). Clearing files from Onboard Memory Storage (БЗУ). Removal of Battery Module No.2 ПТАБ-1М (pos.А302). Sprint Ultrasound Operations First Time – Subject Closing Shutters on windows 6, 8, 9, 12, 13, 14 Sprint Guide Donning Operator Emergency Mask OBT. Glacier Desiccant Swap Alternate USOS Window Shutter Close Test connection of Sony PMW-EX1R and Sony PMW-200 camcorders, Starting NASA MPEG-2 Viewer application. Deactivation of TV System equipment, Crew Onboard Support System (КСПЭ) equipment and closing NASA MPEG-2 Viewer RFID Power Up Fluidics Ending of data copy and T61p Laptop stowage On-board Training (OBT) ISS Emergency Hardware Familiarization Integrated Emergency Hardware Familiarization. ESA ACTIVE DOSIMETER MOBILE UNIT SWAP Meteor Hard Drive Set Swap Electrical Power System Contingency Jumper Installation Big Picture Words Review Terminate Soyuz 733 Samsung PC Battery Charge (as necessary) Downlink data from Sony PMW-EX1R and Sony PMW-200 camcorders via ОСА RFID Power Up Completed Task List Items None Ground Activities All activities were completed unless otherwise noted. OsteoOmics support Fluid Shifts Ultrasound support Three-Day Look Ahead: Thursday, 05/04: OsteoOmics media change, EVA tool config/SAFER checkout, CEVIS portable PFS setup Friday, 05/05: OsteoOmics media change, At Home In Space questionnaire, MARES deploy, Electro-Wetting Saturday, 05/06: Crew off duty, housekeeping QUICK ISS Status – Environmental Control Group: Component Status Elektron On Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) On Carbon Dioxide Removal Assembly (CDRA) Lab Operate Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Operate Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Standby Urine Processing Assembly (UPA) Standby Trace Contaminant Control System (TCCS) Lab Full Up Trace Contaminant Control System (TCCS) Node 3 Off