OsteoOmics: The crew fixated BioCells in Biocell Habitat 1 inserted the sample into the Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI). Crewmembers experience bone loss in orbit stemming from the lack of gravity acting on their bones. OsteoOmics investigates the molecular mechanisms that dictate this bone loss by examining osteoblasts, which form bone, and osteoclasts, which dissolve bone. Improved understanding of these mechanisms could lead to more effective countermeasures to prevent bone loss during space missions and in a wide range of disorders on Earth. This may lead to better preventative care or therapeutic treatments for people suffering bone loss as a result of bone diseases like osteopenia and osteoporosis, or for patients on prolonged bed rest. NanoRack Cubesat Deployer (NRCSD) #12: The crew brought the JEM (Japanese Experiment Module) Airlock (JEMAL) Slide Table (ST) into the ISS and replaced the NRCSD #11 Quad deployers with the NRCSD #12 deployers. The ST was then retracted into the JEMAL which was depressurized and vented. These activities are in preparation for the planned deployment of CubeSats from the NRCSD #12 over the next two days. Grip Commissioning: As part of the ongoing commissioning for the Grip investigation hardware, a crewmember performed tasks in a supine position. Grip tests how the nervous system takes into account the forces due to gravity and inertia when manipulating objects. Results from this investigation may provide insight into potential hazards for astronauts as they manipulate objects in different gravitational environments, support design and control of haptic interfaces to be used in challenging environments such as space, and provide information about motor control that will be useful for the evaluation and rehabilitation of impaired upper limb control in patients with neurological diseases. Fine Motor Skills: The crew completed a series of interactive tasks during a session of FMS which studies how fine motor skills are effected by long-term microgravity exposure, different phases of microgravity adaptation, and sensorimotor recovery after returning to Earth gravity. The goal of the investigation is to determine how fine motor performance in microgravity trends/varies over the duration of a six-month and year-long space mission; how fine motor performance on orbit compare with that of a closely matched participant on Earth; and how performance trends/varies before and after gravitational transitions, including periods of early flight adaptation and very early/near immediate post-flight periods. On-Board Training (OBT) Dragon Rendezvous Review: The crew performed proficiency training on the Dragon mission profile, rendezvous crew procedures, and crew interfaces for monitoring and commanding the vehicle. This training is in preparation for planned SpaceX-11 launch on June 1 and berthing to ISS on June 4. Today’s Planned Activities All activities were completed unless otherwise noted. Reminder 2 CSA Generic Frozen Blood Collection Reminder 2 Fine Motor Skills МО-8. Configuration Setup REMINDER – ESA ACTIVE DOSIMETER MOBILE UNIT SWAP Body Mass Measurement CSA Generic Frozen Blood Collection 25 Minutes – Subject NEUROIMMUNITET. Saliva Sample. Psychological Test (morning) CSA Generic Frozen Blood Collection – Operator KORREKTSIYA. NEUROIMMUNITET. Collection of Blood Samples NEUROIMMUNITET. Venous blood sample processing (smear) KORREKTSIYA. NEUROIMMUNITET. Processing venous blood sample using Plasma-03 centrifuge CSA Generic Refrigerated Centrifuge Configure Insertion of Russian experiments blood samples into MELFI CSA Generic Refrigerated Centrifuge Spin Conclude Environmental Health System (EHS) Potable Water Dispenser (PWD) Sample Collect [Aborted] CSA Generic Sample MELFI Insertion CSA Generic Frozen Blood Collection Conclude And Stow Fine Motor Skills Experiment Test – Subject МО-8. Closeout Ops Fine Motor Skills Experiment Test – Subject MORNING PREPARATION WORK XF305 Camcorder Setup JAXA PCG Canister Bag CBEF Micro-G Installation JEM Airlock Press Routine Maintenance of H2O [КВ], Air Pressure [КН], and Drainage [КД ]Valves in SM Rodnik Tanks Pille Dosimeter Readout after US EVA JEM Airlock Leak Check TV Conference with the Participants of Space Science Week in Izhevsk (Ku + S-band) JEM Airlock Slide Table (ST) Extension to JPM Side Nikon still camera sync with station time NEUROIMMUNITET. Psychological Test NanoRacks CubeSat Deployer Swap ISS Crew departure preparation ISS HAM Radio Power Up ESA Weekly crew conference GRASP Big picture reading OsteoOmics Fixation Operations ZBOOK Hard Drive Installation GRASP additional set-up in seated configuration Environmental Health System (EHS) Coliform Water Processing EHS Total Organic Carbon Analyzer (TOCA) Potable Water Dispenser (PWD) Sample Analysis [Aborted] JEM Airlock Slide Table (ST) Retraction from JPM Side JEM Airlock Depressurization OsteoOmics MELFI Insertion 1 Extravehicular Activity (EVA) Debrief NEUROIMMUNITET. Hair Samples Collection Equipment Stowage under Soyuz 733 center seat. Completion Status Report Note 7 Hardware prep for PAO event. Video and audio signals check Extravehicular Activity (EVA) Air Lock Deconfiguration ESA Monthly Management Conference Inspection and photography of СПА instrumentation block (БА СПА), УС31 and FGB ППС 349 Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record [Aborted] JEM Airlock Vent Audit of RS ISS Docking And Internal Transfer System ССВП items Crew Departure Preparations for Return to Earth JEM Airlock Vent Confirmation On-Board Training (OBT) Dragon OBT Conference On-board Training (OBT) Dragon Rendezvous Review Verification of ИП-1 Flow Sensor Position OsteoOmics Saline Kit Relocate URISIS Hardware Setup On-board Training (OBT) Dragon Robotics Review Completed Task List Items None Ground Activities All activities were completed unless otherwise noted. MT translation from WS6 to WS4 Dragon OBT Support CubeSat Deployer Swap Support Three-Day Look Ahead: Thursday, 05/25: Body Measures, OsteoOmics, GRASP, Dragon RoBOT OBT, OGS Sensor R&R Friday, 05/26: OBT Dragon Offset Grapple, EVA loops scrub/conductivity tests, OBT Soyuz drill, GRASP Saturday, 06/27: Crew off duty, housekeeping QUICK ISS Status – Environmental Control Group: Component Status Elektron Off Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) On Carbon Dioxide Removal Assembly (CDRA) Lab Standby Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Operate Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Standby Trace Contaminant Control System (TCCS) Lab Full up Trace Contaminant Control System (TCCS) Node 3 Off