Rodent Research-5 (RR-5) Systemic Therapy of NELL-1 for Osteoporosis: The crew set up and checked out the spare Rodent Habitat which replaces Rodent Habitat 2 that has excessive condensation in its water box. Later the crew will perform injections of the mice in the Microgravity Science Glovebox (MSG). Spaceflight has significant and rapid effects on the musculoskeletal system; therefore, it is important to investigate targeted therapies that could ameliorate some of the detrimental effects of spaceflight. The NELL-1 drug being studied in the RR-5 investigation has the potential to slow or reverse bone loss during spaceflight. Multi Omics-Mouse: The crew completed initial steps to prepare and check out hardware and facilities to be used for the Multi Omics-Mouse investigation to be performed with rodents arriving on SpaceX-12. They cleaned the Cell Biology Experiment Facility (CBEF), reconfigured video cables between the CBEF and Video Compression and Recording Unit 2 for multiple video channels, installed and set up the Mouse Habitat Unit (MHU) Experiment Laptop Terminal 2, replaced ammonia sensors in the Mouse Habitat Microgravity Interface and 1G units, and installed the Mouse Habitat Unit Back Up Interface Unit. Microbial Tracking-2 (MT-2): The crew collected a saliva sample and placed it in a Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI) in support of the Microbial Tracking-2 investigation. MT-2 monitors the different types of microbes that are present on ISS over a 1 year period through surface and air sampling. After the samples are returned to Earth, a molecular analysis of the RNA and DNA will be conducted to identify the specific microbes that are present on ISS to understand the microbial flora diversity and how it may change over time. NanoRacks Module-52: The crew performed a status check of subexperiments inside NanoRacks Module-52. Photographs were taken of the petri dishes and video will be downlinked. NanoRacks Module-52 is a collection of 6 student-led biological experiments photo-documenting the life-cycle of various molds and bacteria on petri plates in microgravity. Today’s Planned Activities All activities were completed unless otherwise noted. Microbial Tracking-2 Sample MELFI Retrieval Insert Total Organic Carbon Analyzer (TOCA) Water Recovery System (WRS) Sample Analysis JEM Camera Robot Activation Cell Biology Experiment Facility (CBEF) Wipe Previous Mouse breed Synchronization of Nikon Camera with Station Time CBEF Video Cable Reconfiguration to Video Compression and Recording Unit 2 (VRU2) Unpacking of First Priority and USOS Cargo Items from Progress 436 (SM Aft). IMS Update Rodent Habitat 2 Spare Habitat Checkout JEM Fire Indicator Illumination Function Checkout Rodent Habitat 2 Prime to Backup location Rodent Habitat 2 Spare Habitat Setup JEM Camera Robot Deactivation WHC Full Fill NanoRacks Module-52 Status 2 Multi-purpose Small Payload Rack (MSPR) /Group Combustion Module (GCM) Component Deactivation Total Organic Carbon Analyzer (TOCA) Sample Data Record Waste and Hygiene Compartment (WHC) Full Fill Crew Medical Officer (CMO) On Board Training (OBT) Rodent Research Node 2 Camcorder Video Setup Rodent Research 5 Injection 2B Rodent Research MELFI Retrieve 4 PK-4 data hard drives exchange Rodent Research MELFI Retrieve 8 Rodent Research MELFI Retrieve 5 SM Rodnik Water Tank 2 (БВ2) Disinfectant Fill Rodent Research 5 Injection 2A Metal Oxide (METOX) Regeneration Initiation Rodent Research Habitat Stow Mouse Habitat Unit(MHU) Experiment Laptop Terminal 2 (ELT2) Setup Extravehicular Metal Oxide Regenerator Verification Mouse Habitat Unit Interface Unit Ammonia sensor Exchange Mouse Habitat Unit Back Up Interface Unit Installation Hematocrit. Hardware Setup Completed Task List Items ZBook Client Swap (SSC13 – NOD2) Ground Activities All activities were completed unless otherwise noted. ROSA Ops Rodent Research Ops support Three-Day Look Ahead: Friday, 06/23: Microbial Tracking 2 ops, StemCell media change, SPHERES Halo run Saturday, 06/24: Crew off duty, housekeeping Sunday, 06/25: Crew off duty QUICK ISS Status – Environmental Control Group: Component Status Elektron On Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) On Carbon Dioxide Removal Assembly (CDRA) Lab Standby Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Process Trace Contaminant Control System (TCCS) Lab Full up Trace Contaminant Control System (TCCS) Node 3 Off