Cardiac Stem Cells: In support of the ongoing Cardiac Stem Cells investigation, the crew changed out the media in one of the BioCell Habitats in the Microgravity Science Glovebox (MSG). Cardiac Stem Cells investigates how microgravity affects stem cells and the factors that govern their activity, in order to clarify the role of stem cells in cardiac biology and tissue regeneration. This investigation also supports research into the possible acceleration of the aging process in microgravity. Lighting Effects: The crew set up and configured the Light Meter hardware and took readings in the Cupola, which uses the legacy General Luminaire Assembly (GLA). The Lighting Effects experiment hopes to better quantify and qualify how lighting can effect habitability of spacecraft. The light bulbs on the ISS are being replaced with a new system designed for improved crew health and wellness. The Lighting Effects investigation studies the impact of the change from fluorescent light bulbs to solid-state light-emitting diodes (LEDs) with adjustable intensity and color and aims to determine if the new lights can improve crew circadian rhythms, sleep, and cognitive performance. Results from this investigation also have major implications for people on Earth who use electric lights. NanoRacks Module 9: The crew activated mixture tubes in support of the NanoRacks-National Center for Earth and Space Science-Casper (NanoRacks-NCESSE-Casper) investigation. NCESSE supports various schools and student-designed experiments that address challenges of living and working in space. The program is also a key initiative for U.S. science, technology, engineering and math (STEM), that strives to educate and inspire the next generation of scientists and engineers to work on the space program. The specific investigations supported today include Growth and Development of Fathead Minnows in Microgravity, Does the Structure of a Fairy Shrimp Change in Microgravity?, Soybean Germination in Microgravity, and Benefits of Mint. Mobile Servicing System (MSS) Operations: Yesterday afternoon and overnight, Robotics Ground Controllers maneuvered Special Purpose Dexterous Manipulator (SPDM) Arm1 to grasp the Roll Out Solar Array (ROSA) Payload Jettison Micro-Square Fixture (MSF). At 4:15pm CDT Controllers applied power to the ROSA Jettison Motors and the ROSA Payload was jettisoned from ISS. Next they maneuvered the Space Station Remote Manipulator System (SSRMS) and SPDM to release Arm1 from the ROSA Jettison MSF to grasp the ROSA Flight Releasable Attachment Mechanism (FRAM) MSF with Arm1 and to release Arm2 from the ROSA Operations MSF. Finally the SSRMS and SPDM were maneuvered to position the ROSA FRAM at the Dragon Trunk threshold in preparation for stowing it later today. Potable Water Dispenser Relocation: The crew installed a potable water tee on the Node 1 to Node 3 portion of the potable water hose to provide a connection for the PWD. The crew then transferred the PWD from ExPRESS-6 to the galley rack. Commercial Orbital Transport Services (COTS) UHF Communication Unit (CUCU) Checkout: In preparation for the upcoming SpaceX-11 departure planned for July 2nd, the crew coordinated with ground teams to activate the CUCU System and perform a Crew Command Panel (CCP) checkout. CUCU provides a command and telemetry communications link between ISS and Dragon during free flight operations in the vicinity of ISS. Today’s Planned Activities All activities were completed unless otherwise noted. Test Video Recording for Russia Today TV Channel Preparation of Reports for Roscosmos Web Site and Social Media URAGAN. Observation and Photography ECON-M. Observation and Photography ARED Photo/TV Reminder Glacier 4 Sample Remove Total Organic Carbon Analyzer (TOCA) Water Recovery System (WRS) Sample Analysis Replacement of sleeves and leg shells on ORLAN-МК #6 – prep ARED Quarterly Inspection Crew Medical Officer (CMO) Proficiency Training ORLAN-МК #6 sleeves R&R Lighting Effects Light Meter Readings Alternate 1 СК ОРЛАН-МК №6 sleeves replacement – help European Modular Cultivation System Gas Valve Close Environmental Health System (EHS) – Formaldehyde Monitoring Kit (FMK) Deployment Operations Environmental Health System (EHS) Grab Sample Container (GSC) Sampling Operations Dragon Lithium Hydroxide Filter Bag Remove and Replace ORLAN-МК #6 leak check. Countermeasures System (CMS) Heart Rate Monitor (HRM) iPad Downlink Cardiac Stem Cells SABL Insertion Cardiac Stem Cells Media Change 1 BioCell Habitat WHC Pre-Treat Tank Remove & Replace Progress 435 (DC1) stow ops for the hardware to be disposed of, with IMS update Photo T/V (P/TV) Advanced Resistive Exercise Device (ARED) Exercise Video Setup Node 2 Nadir Vestibule Outfitting Kit (VOK) Gather NanoRacks Module 9 Ops Session 4 Galley Potable Water Dispenser (PWD) Installation Photo/TV Camcorder Setup Verification TOCA Sample Data Record Photo/TV Camcorder Setup Verification Crew Medical Officer (CMO) Proficiency Training Photo/TV CUCU Video Setup COTS UHF Communication Unit (CUCU) Crew Command Panel (CCP) Checkout Photo TV CUCU Video Deactivation Telescience Resource Kit Laptop Setup Completed Task List Items Crew powers down, unplugs, and stows Fast Neutron Spectrometer hardware Ground Activities All activities were completed unless otherwise noted. SSRMS Ops for ROSA FRAM Install into Drago Trunk CUCU Checkout Three-Day Look Ahead: Wednesday, 06/28: ELF sample holder exchange, sample transfer from MELFI to POLAR, WHC pre-treat tank R&R, Rodent Habitat restock Thursday, 06/29: NanoRacks Module 48 ops, Rodent Research, Dragon departure OBT Friday, 06/30: NanoRacks Module 9 ops, POLAR transfer to Dragon, StemCell media change QUICK ISS Status – Environmental Control Group: Component Status Elektron On Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) On Carbon Dioxide Removal Assembly (CDRA) Lab Standby Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Process Trace Contaminant Control System (TCCS) Lab Full up Trace Contaminant Control System (TCCS) Node 3 Off