Extremophiles: In preparation for Session C of the Extremophiles investigation the crew wiped designated surfaces in Node 1, Node 3 and the Cupola with sterile wet wipes and placed them into a Biolab Thermal Control Unit. Archaea and extremophilic bacteria have not been considered as significant contributors to the microbiome on the ISS. The Extremophiles experiment will add critical knowledge about the microbial diversity on the ISS. The experiment will Isolate and characterize archaea and extremophilic bacteria by sampling selected locations inside the Station. Changes in archaea and extremophilic bacteria over a period of at least 3 months will be assessed. The population of archaea and extremophilic bacteria on the ISS will be compared with that of spacecraft clean rooms and visiting vehicles. Rodent Research-5 (RR-5) Systemic Therapy of NELL-1 for Osteoporosis: The crew reviewed material on live animal return operations and discussed those operations with the Payload Developer. They then set up the Animal Transporter for return of 10 RR-5 mice on SpX-11, powered up the Transporter, installed food bars and activated water lixits. Because spaceflight has significant and rapid effects on the musculoskeletal system, it is important to investigate targeted therapies that could ameliorate some of the detrimental effects of spaceflight. The NELL-1 drug being studied in the RR-5 investigation has the potential to slow or reverse bone loss during spaceflight. NanoRacks Module-52: The crew performed a status check of subexperiments inside NanoRacks Module-52. Photographs and video were taken of the petri dishes. NanoRacks Module-52 is a collection of 6 student-led biological experiments photo-documenting the life-cycle of various molds and bacteria on petri plates in microgravity. NanoRacks Module-48: The crew took photos for the NanoRacks Module-48 investigation for downlink to the ground. NanoRacks Module-48 connects students on Earth to the space program by sending their photographs and messages to the ISS along with plant seeds that are germinated after being returned to Earth. The investigation increases awareness of humans’ ability to access space, spurring interest in the space program and encouraging students to pursue careers in science, technology, engineering, and math. On Board Training (OBT) Dragon Robotics On Board Trainer (RoBOT): In preparation for SpX-11 unberth and release currently planned for Sunday, July 2, the crew completed this 70-minute training session during which they practiced 2 Dragon release runs. They also reviewed departure monitoring and procedures to prevent loss of/recovery of attitude control in the event of a loss of comm with ground teams during free-flyer release. SpaceX (SpX)-11 Cargo Operations: The crew completed approximately 57 hours of cargo operations with approximately 5 hours of packing remaining. SpX-11 is scheduled to unberth on July 2nd, 2017. Today’s Planned Activities All activities were completed unless otherwise noted. Health Maintenance System (HMS) Periodic Health Status (PHS) Evaluation Setup JEM Airlock Depressurization Health Maintenance System (HMS) Periodic Health Status (PHS) Evaluation Health Maintenance System (HMS) Periodic Health Status (PHS) Stow Hardware NanoRacks Module-52 Status R&R of Onboard Heating Unit (БПО) (1 ea.) in Vozdukh NanoRacks Module-48 Imagery R&R of Condensate Water Processor [СРВ-К2М] Hoses Extremophiles Session C JEM Camera Robot Cable Swap Extremophiles Photo Shooting Formaldehyde Monitoring Kit (FMK) Stow Operation Rodent Research 5 ARS Review Rodent Research 5 Live Animal Return Review Rodent Research Crew Conference Rodent Research 5 Transporter Setup JEM Airlock Vent Crew Medical Officer (CMO) Proficiency Training CO Partial Pressure Measurement at the Central Post using USOS CSA-CP Gas Analyzer ROBoT Software Startup JEM Airlock Vent Confirmation MSPR Combustion Chamber (CC) Valve 2 Operation [Aborted] On-board Training (OBT) Dragon Departure Review Cargo Transfer from 67P (SM Aft). IMS Update On-board Training (OBT) Dragon Robotics Onboard Trainer (ROBoT) Release Combustion Integrated Rack Alignment Guide Install Completed Task List Items Genes in Space Biomolecule Sequencer Stop Ground Activities All activities were completed unless otherwise noted. Rodent Research Transporter setup JEMAL depress/vent monitoring OBT – Dragon RoBOT Release Three-Day Look Ahead: Friday, 06/30: NanoRacks Module 9 ops, POLAR transfer to Dragon, StemCell media change Saturday, 07/01: Crew off duty, housekeeping Sunday, 07/02: SpX-11 unberth QUICK ISS Status – Environmental Control Group: Component Status Elektron On Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) On Carbon Dioxide Removal Assembly (CDRA) Lab Standby Carbon Dioxide Removal Assembly (CDRA) Node 3 Operate Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Process Trace Contaminant Control System (TCCS) Lab Full up Trace Contaminant Control System (TCCS) Node 3 Off