Node 3 Common Cabin Air Assembly (CCAA) Water Separator (WS) Change Out: Today, the crew replaced the Node 3 CCAA WS. The unit had experienced several water carryover events since May 3, 2017. To assist in preventing these events, ground teams adjusted the Node 3 Low Temperature Loop to reduce condensation rates. The failing unit esd operational for over 7 years with expected life of 5 years. Ground teams activated the newly repaired CCAA and are receiving good telemetry. Magnetic 3 Dimensional (Mag 3D) Cell Culturing: With assistance from the Payload Developer, the crew used a microscope to view Magnetic 3D Biocells. They fixated the biocells and inserted them in to a Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI). This was followed by injections of Magnetic 3D cultures media into the multiwell Biocells. Cell cultures in space spontaneously grow in three dimensions, resulting in characteristics more representative of how cells grow and function in living organisms. But in microgravity, routine manipulation of cell cultures is challenging. This investigation uses magnetized cells and tools to make it easier to handle cells and cultures and to improve the reproducibility of experiments. This approach also makes it possible to generate two-dimensional cultures as controls, and to determine whether biological events in these monolayer cultures result from gravity or substrate attachment. Microbial Monitoring System (MMS): The crew configured the hardware and collected low and high DNA concentration deionized water samples using the Razor Polymerase Chain Reaction (PCR) Microbial Monitoring System. They then transferred data from the sample tests for downlink. The MMS supports crew testing and monitoring of the safety of water supplies on the ISS and is also applicable to future space missions where Earth-based testing would be difficult or impossible. Habitability Walk-through: The crew will record and submit a walk-through video today documenting observations of life onboard ISS and providing insights related to human factors and habitability. The investigation collects observations about the relationship between crew members and their environment on the ISS. These observations can help spacecraft designers understand habitable volume requirements and whether a mission’s duration impacts how much space crewmembers need or not. Today’s Planned Activities All activities were completed unless otherwise noted. Extremophiles Sampling Session B Наклейка русских букв на клавиатуру лэптопа RSК2 VIZIR. Modification of SM panels 114, 116 for СКПФ-УМ P/L URAGAN. Observation and Photography ECON-M. Observation and Photography Calf Volume Measurement Virus Definition File Update on Auxiliary Computer System [ВКС] Laptops CCAA Water Separator R&R Magnetic 3D Cultures MELFI Operations Acoustic Dosimeter Setup Photography of SM SKK cartridge No.9 Combustion Integrated Rack Alignment Guide Install WORF IPEHG Shutter Actuator System Procedure Review Study of veins in lower extremities Magnetic 3D Cultures Microscope Operations Replacement of RSK2 Laptop Shell. HRF1 PC 1 USB Load Installation [Aborted] Search for equipment and procedure review to replace [СКВ1] compressor. Magnetic 3D Cultures Biocells Fixation URAGAN. VSS P/L SW Adjustment and Test Imagery. Glacier Desiccant Swap Alternate Verification of ИП-1 Flow Sensor Position MELFI Ice Brick Insert Auxiliary Computer Equipment Audit Analysis of SM Atmosphere for Freon Using Freon Leak Analyzer/Detector (ФИТ) Health Maintenance System (HMS) Spaceflight Cognitive Assessment Tool for Windows (WinSCAT) Test Magnetic 3D Cultures Inoculation WORF IPEHG Shutter Actuation System Conference MELFI Rodent Research Transfer Max Cycle Ergometer w/ Vibration Isolation & Stabilization (CEVIS) Portable PFS Partial Set Up Metal Oxide (METOX) Regeneration Habitability Walk-through Video Increment 51 Plaque Hanging Completed Task List Items Microbial Monitoring System On-Board Training Microbial Monitoring System Setup, Preparation, and Sampling Ground Activities All activities were completed unless otherwise noted. Node 3 CCAA Activation Post R&R Three-Day Look Ahead: Wednesday, 07/12: Max CEVIS session, TPF experiment setup, ELF sample holder exchange, RR Habitat restock Thursday, 07/13: WORF PEHG/Shutter actuator system install, JEMRMS cable reconfig for BDS checkout, RR access unit cleaning Friday, 07/14: Fluid Shifts, JEM Small Satellite Orbital Deplorer (SSOD) Removal, Optical Coherence Tomography