Researchers have developed a high-speed cell sorting method of large cells with high-viability using dual on-chip pumps. The microfluidic chip has three-branched microchannels. Target cells are sorted into one of two interest channels by the high-speed flow produced by the on-chip pumps, while non-target cells enter a waste channel without pump actuation. The technique overcomes the limitation of many on-chip cell sorting methods in achieving the sorting of large cells at a high throughput.