Multi-Omics Sample Collections: A 52S crewmember collected fecal samples for the Japan Aerospace Exploration Agency (JAXA) Multi-Omics experiment. The samples were placed into the Minus Eighty Degree Celsius Laboratory Freezer for ISS (MELFI). The Multi-omics analysis of human microbial-metabolic cross-talk in the space ecosystem (Multi-Omics) investigation evaluates the impacts of space environment and prebiotics on astronauts’ immune function, by combining the data obtained from the measurements of changes in the gut microbiological composition, metabolites profiles, and the immune system. Veg-03 Operations: The crew photodocumented the status of the plants in the Veggie facility, and evaluated the plant pillows to determine if additional watering is required. The Veg-03 investigation uses the Veggie plant growth facility to cultivate a type of cabbage, lettuce and mizuna which are harvested on-orbit with samples returned to Earth for testing. Organisms grow differently in space, from single-celled bacteria to plants and humans. Future long-duration space missions will require crew members to grow their own food, so understanding how plants respond to microgravity is an important step toward that goal. Veg-03 uses the Veggie plant growth facility to cultivate a type of cabbage, lettuce and mizuna which are harvested on-orbit with samples returned to Earth for testing. USOS Extra Vehicular Activity (EVA) 45 Preparation: Today the crew reconfigured necessary EVA tools, reviewed procedures, and participated in a conference with ground EVA planners in support of next week’s EVA. The goals of this EVA include: Removal and Replacement of the degraded CP9 camera and lubrication of the Latching End Effector (LEE) B. External Robotics Operations: Over the weekend, robotics ground controllers walked the Space Station Remote Manipulator System (SSRMS) onto Mobile Base System (MBS) Power Data Grapple Fixture 3 (PDGF3). They then maneuvered SSRMS as required to perform a video and imagery survey of the Latching End Effector B (LEE-B) snare cables using an External High Definition Camera (EHDC). Once the survey was completed, the SSRMS was walked off onto MBS PDGF4 in preparation of US EVA 45 scheduled tomorrow (GMT 283). Today’s Planned Activities All activities were completed unless otherwise noted. TORU OBT. Tagup with Instructor Multi Omics Fecal Sample Operations KUBIK 6 Setup Multi Omics Fecal Sample MELFI Insertion Columbus WOOV9,10 Inspection Adjustment of KUBIK sensor signal to e-box US Extravehicular Activity (EVA) Tether Inspection KUBIK Temperature Measurement Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Water Recovery System (WRS) Sample Analysis Extravehicular Activity (EVA) Tool Configuring BIOCARD. Experiment Session Assistance BIOCARD. Experiment Session VEG-03 Plant Photo Extravehicular Activity (EVA) Tool Audit Progress 436 [AO] Cargo Transfers and IMS Ops Extravehicular Activity (EVA) Procedure Review PELLE Data Download & deployment before EVA MOTOCARD. Experiment Session Extravehicular Activity (EVA) Node 1 Vacuum Access Port (VAP) Check Regenerative Environmental Control and Life Support System (RGN) Wastewater Storage Tank Assembly (WSTA) Fill ЕДВ Inspection Environmental Health System (EHS) Total Organic Carbon Analyzer (TOCA) Sample Data Record MOTOCARD. Photography of “Diasled” holster and БП-015 unit СОЖ maintenance Extravehicular Mobility Unit (EMU) Cuff Checklist Print Equipment Lock (E-LK) Preparation Contingency Water Container (CWC) Locate Extravehicular Activity (EVA) Procedure Conference Exercise Data Downlink via OCA Completed Task List Activities Emergency Mask OBT Questionnaire ESA-PAO-CABOT ESA-PAO-CPR ESA-PAO-EUINST ESA PAO Recorded Message Science Festival EVA BAYONET RECEPTACLE CLEAN Extravehicular Glove Inspection Health Maintenance System (HMS) Spaceflight Cognitive Assessment Tool for Windows (WinSCAT) Test Printer Troubleshoot VEG-03 Plant Pillow Water VEG-03 Plant Pillow Water WHC Trash Photograph Ground Activities All activities were completed unless otherwise noted. CDRA blower fan speed test Standard commanding Three-Day Look Ahead: Tuesday, 10/09: US EVA 45 Wednesday, 10/11: Urine Transfer, ACME Chamber Install and Config, Neuromapping, HAM Radio Ops Thursday, 10/12: CIR Ops and ACME Config, WHC Dose Pump R&R, EVA 46 Tool Config QUICK ISS Status – Environmental Control Group: Component Status Elektron On Vozdukh Manual [СКВ] 1 – SM Air Conditioner System (“SKV1”) Off [СКВ] 2 – SM Air Conditioner System (“SKV2”) Off Carbon Dioxide Removal Assembly (CDRA) Lab Operate Carbon Dioxide Removal Assembly (CDRA) Node 3 Standby Major Constituent Analyzer (MCA) Lab Idle Major Constituent Analyzer (MCA) Node 3 Operate Oxygen Generation Assembly (OGA) Process Urine Processing Assembly (UPA) Standby Trace Contaminant Control System (TCCS) Lab Full Up Trace Contaminant Control System (TCCS) Node 3 Off