Proteins are the nano-machines that Nature uses to perform most of the processes critical for the metabolism in cells. One of the key goals of life and physical sciences revolves around understanding the structural and dynamic properties of the native, transition, intermediate, and denatured states of proteins. The denaturation transition – defined as the transition of proteins from their specific native functional state to the unfolded inoperative state – is of particular interest, as it is defining the boundaries of stability and functionality of the phase diagram of proteins.