Abstract: In 2017, the combined Optical Telescope Element and Integrated Science Instrument Module (OTIS) of the James Webb Space Telescope (JWST) underwent functional testing and optical metrology verification under cryogenic vacuum conditions in Chamber A at the Johnson Space Center (JSC). Testing the infrared Science Instruments and OTIS optics below 50 degrees Kelvin required an environment architecture that comprehensively controlled the temperature and energy path of every seam and penetration in…