Abstract: Anomalously fractionated isotopic material is found in many primitive Solar System objects, such as meteorites and comets. It is thought, in some cases, to trace interstellar matter that was incorporated into the Solar Nebula without undergoing significant processing. We will review observations and models of the nitrogen, oxygen, and carbon fractionation chemistry in dense molecular clouds. The range of fractionation ratios expected in different interstellar molecules will be discussed and c…