A species of globally recognised medicinal mushroom was recorded for the first time in Thailand. Commonly referred to as lingzhi, the fungus (Ganoderma tropicum) was collected from the base of a living tree in Chiang Rai Province, Northern Thailand. Additionally, the study reports the first assessment of the optimum conditions needed for the species to grow its mycelia (the vegetative part of a fungus consisting of a branching network of fine, thread-like structures) and spread its colony.