CRISPR-Cas9 is a powerful, high-throughput gene-editing tool that can help scientists engineer organisms for bioenergy applications. Cas9 needs guide RNA to lead it to the correct sequence to snip—but not all guides are effective. To ensure the gene editing tool will make the right cut, researchers created a set of guide RNAs that were effective against 94 percent of the genes in a lipid-prolific yeast.
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Source: Phys.org