Today’s state-of-the-art analysis of biological samples by light microscopy includes a vast variety of techniques ranging from conventional bright field microscopy and phase contrast microscopy to high resolution confocal laser scanning microscopy and to recently developed super resolution microscopy techniques like stimulated emission depletion (STED) or stochastic optical reconstruction microscopy (STORM) which abnegate Abbe’s limit of diffraction.
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Source: Phys.org