Accurately reconstructing the relationships between different species requires analyzing the sequences of a judiciously selected, and preferably large, sample of different genes. Hybrid capture with high-throughput sequencing, or Hyb-Seq, is a powerful tool for obtaining those gene sequences, but must be calibrated for each group analyzed to ensure an informative sample of genes are sequenced. Researchers must take a variety of considerations into account when selecting which genes to sequence, and the choices made in gene sampling can affect the outcome of the analysis. In work presented in a recent issue of Applications in Plant Sciences, Dr. Katy Jones and colleagues evaluated the performance of a Hyb-Seq probe set designed for the large and diverse sunflower family, Asteraceae, and found it to be effective in reconstructing relationships at multiple taxonomic levels, from subspecies to tribe.
Click here for original story, Understanding Asteraceae: Validation of a Hyb-Seq probe set for evolutionary studies
Source: Phys.org