A team of scientists from the Leibniz Institute for Zoo and Wildlife Research (Leibniz-IZW), the Australian Museum and the Max Delbrück Center for Molecular Medicine (MDC) report a new method for identifying any genome sequence located next to a known sequence. It is often difficult to precisely determine unknown sequences close to small known fragments. Whole genome sequencing can be a solution, but it’s a very cost intensive approach. In order to find a more efficient technique, the scientists developed Sonication Inverse PCR (SIP): First, DNA is cut into random pieces using ultrasound waves. After DNA fragmentation, long-range inverse PCR is performed followed by long-fragment high-throughput sequencing. SIP can be used to characterize any DNA sequence (near a known sequence) and can be applied across genomics applications within a clinical setting as well as molecular evolutionary analyses. The results are reported in the scientific journal Methods in Ecology and Evolution.
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Source: Phys.org