The latest developments in fluorescence microscopy make it possible to image individual molecules in cells or molecular complexes with a spatial resolution of up to 20 nanometres. However, under certain circumstances, an effect occurs that falsifies the results: the laser light used can cause very reactive oxygen molecules to form in the sample. These can then damage the fluorescent dyes used to such an extent that they no longer fluoresce. Among microscopy experts, this effect is known as photobleaching.
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Source: Phys.org