Recent advances in imaging technology have made it possible to visualize intracellular dynamics, which offers a better understanding of several key biological principles for accelerating therapeutic development. Fluorescent labeling is one such technique that is used to identify intracellular proteins, their dynamics, and dysfunction. Both internal as well as external probes with fluorescent dyes are used for this purpose, although external probes can better visualize intracellular proteins as compared to the internal probes. However, their application is limited by non-specific binding to intracellular components, resulting in a low target specific signaling and higher background noise.
Click here for original story, Researchers report on live intracellular imaging with new, conditionally active immunofluorescence probe
Source: Phys.org