Sau3AI is a type II restriction enzyme widely used for genetic manipulation, such as genome library construction. Sau3AI consists of two domains, the N-terminal domain (Sau3AI-N) and the C-terminal domain (Sau3AI-C). How these two domains work together to cut DNA remains unclear.
Click here for original story, Researchers determine how type II restriction endonuclease Sau3AI cleaves DNA
Source: Phys.org