The mechanical properties of cells have an impact on biological processes ranging from wound healing and disease to cellular aging and differentiation. Currently, the most popular method of measuring the mechanical properties of a cell is by atomic-force microscopy (AFM). Very simply, AFM works by moving a very fine needle attached to a cantilever beam across the sample surface, and the deflection of the beam is measured directly with a laser. Very high resolution (< nanoscale) measurements are possible with this method, and it is used for imaging, force measurement, and fine manipulation.