Abstract: Absolute calibration of radiometers is implemented onboard using one hot and one cold external calibration targets. However, two-point calibration methods are unable to differentiate calibration drifts and associated errors from fluctuations in receiver gain and offset. This paper investigates the use of onboard three-point calibration algorithm for microwave radiometers to track calibration drifts and characterize associated errors in Earth and Space measurements of the radiometer.