Conventional wide-field microscopy (WFM) cannot provide optical sectioning (OS) images that are required for 3-D volumetric reconstruction. The reason lies in the fact that the out-of-focus signals are always coupled within the in-focus plane. By introducing structured illumination microscopy (SIM), researchers have achieved removing the out-of-focus components from the in-focus plane in full color (FC).
Click here for original story, Researchers directly obtain 3-D, full-color images with conventional microscope
Source: Phys.org